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Image Search Results
Journal: Cell Insight
Article Title: LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability
doi: 10.1016/j.cellin.2024.100170
Figure Lengend Snippet: Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Article Snippet: Statistical analysis was performed using
Techniques: CCK-8 Assay, Flow Cytometry, Western Blot, Immunofluorescence, Fluorescence, Staining, Software, Immunostaining