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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Histograms Graphpad Prism 8.01, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Graphpad Prism 8, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
8.01 Software, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Jmp Statistical Analysis Software, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Statistical Software Jmp, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Software Packages Sas 8.01, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anova+statistical+software+sas+version+8%2E01/pm15741985-78-6-7?v=SAS+institute
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software packages sas 8.01 - by Bioz Stars, 2026-08
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SAS institute statistical software version 8.01
Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Statistical Software Version 8.01, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anova+statistical+software+sas+version+8%2E01/pm15939852-69-1-5?v=SAS+institute
Average 90 stars, based on 1 article reviews
statistical software version 8.01 - by Bioz Stars, 2026-08
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SAS institute statistical analysis system software release 8.01
Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.
Statistical Analysis System Software Release 8.01, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistical analysis system software release 8.01 - by Bioz Stars, 2026-08
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Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.

Journal: Cell Insight

Article Title: LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability

doi: 10.1016/j.cellin.2024.100170

Figure Lengend Snippet: Impact of LUC7L3 depletion on cell proliferation A. Knockdown of LUC7L, LUC7L2, LUC7L3, and RBM25 proteins in A549 and HeLa cell lines. Cell viability was assessed using the CCK-8 assay. B. Flow cytometry analysis of apoptosis in LUC7L3-interfered A549 and HeLa cells. C. Detection of senescence-associated β-galactosidase (SA-β-Gal)-positive cells in LUC7L3-interfered RPE, A549, and HeLa cells using a cellular senescence kit. D. Western blot analysis of γH2AX, KAP1, and p-KAP1 (S824) protein levels in A549 cells following knockdown of LUC7-like proteins LUC7L, LUC7L2, LUC7L3, and RBM25. E. Immunofluorescence analysis of γH2AX nuclear foci in LUC7L3-knockdown A549 and HeLa cells. Red fluorescence indicates γH2AX, and blue indicates the nucleus with DAPI staining. The number of γH2AX foci per cell was analyzed using Image J software and statistical analysis was performed using GraphPad Prism 8.0.1 software. n>70. F. Immunostaining of intracellular micronuclei using DAPI in LUC7L3-depleted A549 and HeLa cells. Red arrow: micronuclei. Statistical analysis of the number of micronuclei cells was shown. n>100. G. Anaphase cells of LUC7L3 deleted A549 cells were immunostained with DAPI. Statistical analysis of the number of nucleoplasmic bridge cells was shown. Red arrow: chromosome bridge. n>100. H. Immunostaining of multinuclear cells with DAPI in LUC7L3-depleted cells. Dashed red circle: multinuclear cells. Statistical analysis of the number of multinucleated cells was shown. n>100. ∗p ​< ​0.05, ∗∗p ​< ​0.01, ∗∗∗p ​< ​0.001, ∗∗∗∗p ​< ​0.0001, indicating statistical significance and ns indicates non-significance. Scale bar: 10 ​μm. Dash lines outlined cells with micronucleus or multinuclear cells.

Article Snippet: Statistical analysis was performed using GraphPad Prism 8.0.1 software.

Techniques: CCK-8 Assay, Flow Cytometry, Western Blot, Immunofluorescence, Fluorescence, Staining, Software, Immunostaining